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Plasmodium falciparum : a simplified technique for obtaining singly infected erythrocytes

Identifieur interne : 001F68 ( Main/Exploration ); précédent : 001F67; suivant : 001F69

Plasmodium falciparum : a simplified technique for obtaining singly infected erythrocytes

Auteurs : Manoj K. Puthia [Singapour] ; Kevin S. W. Tan [Singapour]

Source :

RBID : ISTEX:7E582B68F55158E7675EB1D5DEB15AAC98396E7F

Abstract

Abstract: We report the development of a simple technique involving 15 ml polypropylene tubes and a rotatory incubator for obtaining erythrocytes singly infected with Plasmodium falciparum. This technique will be useful for cloning of the parasite. Our finding that P. falciparum merozoite invasion is inhibited during rotation suggests that this method may also be useful for the study of parasite-erythrocyte interactions under dynamic circulatory conditions.

Url:
DOI: 10.1007/s00436-004-1266-6


Affiliations:


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